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Cells were deprived of growth factors by incubation in starvation media RPMI-1640 containing 0.5% (w/v) BSA (Sigma, St Louis, MO, USA) for 18 h before stimulation experiment with HGF (40 ng ml 1 for 7.5 min)

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An increase in the activity of matrix-metalloproteases (MMPs) or an increase in neuronal glucose uptake are two likely mechanisms by which inhibition of IRAP can provide an enhancement of memory