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GHK-Cu attracts immune cells (macrophages, mast cells) and endothelial cells to injury sites, stimulates angiogenesis (new blood vessel formation), and promotes nerve outgrowth

Cells in the HG condition were cultured in the medium containing 35 mM of D-glucose (Sigma-Aldrich), and cells in the SG condition were cultured in DMEM/F12 (this represents control, CTR, condition)

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These effects were mediated through downstream activation of transcription factors including c-Fos, c-Jun, and EGR-1, which regulate genes involved in cell cycle progression, extracellular matrix remodelling, and angiogenic signalling