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doi: 10.1038/cr.2010.178 98 PasparakisM

Either should be just fine, though doing it multiple times still needs you to follow sterile procedures each time

to allow the MTS to react with the metabolic products of the adherent cells before reading in a SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA) at an absorbance wavelength of 490 nm

miR-34a deletion and EGFR amplification are linked to poor survival, whereas high miR-340 and miR-615 expression are correlated with longer overall and recurrence-free survival, 82,83 reinforcing their potential as independent prognostic factors