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Description

Cell-type targeted NF-kappaB inhibition for the treatment of inflammatory diseases

Induction of ferroptosis and mitochondrial dysfunction by oxidative stress in PC12 cells

The next day, cells were transfected with siRNA by combining 10l of 20M siRNA and 5L Dharmafect (Dharmacon, T-2001-03) in 800L OptiMEM (Gibco, 31985-062) and adding to a 6-well plate in RPMI media to a total volume of 4mL, for a final siRNA concentration of 50nM