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This complementary mechanism preserves natural feedback loops while significantly amplifying growth hormone secretion, distinguishing it from continuous, non-pulsatile stimulation approaches that may lead to receptor desensitization

Beads were sequentially washed in 500-l solutions of: 1x 1% SDS in 50 mM HEPES, 1x 500 l 0.2% SDS in 50 mM HEPES, 1x 500 l 0.2% SDS, 40 mM CAA, 10 mM TCEP in 50 mM HEPES (10-min incubation with agitation) and finally 2x 500 l 50 mM HEPES

The potential relationship between heavy metal exposure and homocysteine is discussed later in this report

Carlo Gabriele Tocchetti, orcid.org/0000-0001-5983-688X