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Mechanistically, the inflammatory human caspase-1/4/5 and mouse caspase-1/11 as well as caspase-3, serine protease, mediate proteolytic cleavage of corresponding gasdermins (GSDMs) and liberate N-terminal (NT) fragments binding to phospholipids in inner plasma membrane to form pores, enabling the release of pro-inflammatory cytokines and plasma membrane rupture 5,6,7,8

Puncture the stopper of the bacteriostatic water vial and inject the air

Aggregation happens when peptide molecules stick together, forming larger complexes that may precipitate from solution or lose biological activity

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