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As glycine acts as a powerful inhibitory neurotransmitter in the brain and spinal chord [108] and glycine transporters are differentially expressed throughout the CNS [109], it may be relevant for future studies of children with autism spectrum disorders to examine whether there are abnormalities in expression of glycine transporters in the CNS or in glycine transport across the choroid plexus in children with autism spectrum disorders

Common pitfalls with cell viability assays are: Assays lack sensitivity threshold required for reproducibility and high-throughput screening Lacking an option for kinetic monitoring Viability assays measure how many cells are alive, but not whether theyre actively dividing

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