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Maintenance doses are typically 1 mg every 2-3 months for life High-dose oral therapy (1000-2000 micrograms daily) may be effective in selected patients with pernicious anaemia under clinical supervision, as passive diffusion can compensate for absent intrinsic factor, though this is not routine first-line treatment in the UK The water-soluble nature of B12 means that high-dose supplementation carries minimal risk of toxicity , making it a safe option for individuals with confirmed or suspected deficiency

Numerous studies have shown that vitamin D receptor plays a positive role by inhibiting ferroptosis [79,80,81]

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Therefore, elucidating these unknown microbiota-metabolite interactions will not only reveal fundamental mechanisms governing ISC functionality but also provide actionable targets for therapeutic interventions aimed at enhancing epithelial regeneration in barrier disorders