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But to do this, it needs certain nutrients as building materials, including the aforementioned amino acids: cysteine, glycine, and glutamic acid

Critically, in vitro binding or degradation does not guarantee efficacy in vivo due to the complex gastrointestinal environment, including variable pH, proteolytic enzymes, bile salts, and competition from resident microbiota (30, 94)

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For the analysis of PTP oxidation, frozen liver samples were homogenised under anaerobic conditions in the presence of N -ethylmaleimide to alkylate all reduced PTPs and then subsequently reduced and hyperoxidised to the sulfonic state as described previously [13]