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Experimental evidence suggests that the expression of GPx-2 and GPx-4 is less sensitive to variations in selenium levels compared to GPx-1 or GPx-3 (see section III)

Zhou N, Feldman HA, Corsini EM, Antonoff MB , Mehran RJ, Rajaram R, Rice RC, Roth JA, Sepesi B, Swisher SG, Vaporciyan AA, Walsh GL, Ajani JA, Hofstetter WL

This assay was performed on MS medium for the convenience of identifying a clear phenotype, which will be difficult on CIM as callus induction and/or growth might interfere with the phenotype