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Real-time qPCR Total RNA was isolated with TRIZOL reagent (Invitrogen) and reverse transcription was performed by ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO), according to the manufacturers protocol

Jardieu P, Clark R, Mortensen D, Dorshkind K: In vivo administration of insulin-like growth factor-I stimulates primary B lymphopoiesis and enhances lymphocyte recovery after bone marrow transplantation