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A post-hoc analysis suggested that oral lipoic acid supplementation may reduce neuropathic symptoms particularly in subjects with a high burden of cardiovascular disease, diabetes, and neuropathy yet with normal body mass index (BMI) and blood pressure (79)

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Other peptides under investigation Several additional peptides are being studied for disc-related applications, though with less established evidence: Vasoactive Intestinal Peptide (VIP) has demonstrated the ability to slow disc degeneration progression and improve aggrecan levels in mouse studies

After different groups of HUVECs are treated accordingly, they are seeded into 24-well plates pre-coated with 150 L/well growth factor-reduced Matrigel (Corning, catalog number 354234) and incubated at 37 C in a cell culture incubator for 12 h