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Freezethaw: Avoid repeated freezethaw cycles of the reconstituted solution

Rabbit monoclonal antibodies against HSP90, TGF, and -actin (dilution 1:1000) were added and incubated overnight at 4C

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Subsequent knockdown of CACNA1A resulted in the observation of characteristic ferroptotic morphological changes under electron microscopy, alongside alterations in typical ferroptosis-related metabolic indicators