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The column employed was a Kinetex 1.7 m EVO C18 50 mm x 2.1 mm (Phenomenex, USA) with a quaternary pump equipped with the Agilent 1260 Infinity HPLC Series (Agilent, USA)

Since it was founded in 1999, Niagen (NR) has undergone 175+ peer-reviewed preclinical studies to validate its mechanisms and efficacy

Generation and quantification of protein persulfides and polysulfides in rat heart lysates using Na 2 S 2 Female Wistar rats (obtained from Charles River Laboratories, UK) were housed in pathogen-free facilities with ad libitum access to standard chow and water and were used for experiments at 912 weeks of age