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Analytical evaluation typically includes: Identity Confirmation: Verification of the peptide sequence and structure

To minimize non-specific binding, the membrane was then blocked with 5% bovine serum albumin (BSA) for a duration of 1 h at ambient temperature

The reaction catalyzed by GPATase, being the first committed step of the pathway, represents the primary control point

Moreover, HAs strong hydrophilicity, while advantageous for tissue hydration and lubrication, often results in weak mechanical strength, poor structural stability, and rapid clearance, limiting its use in load-bearing tissues or long-term applications