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TSH influences serum leptin levels independent of thyroid hormones in hypothyroid and hyperthyroid patients

After boiling, 450 uL of ice cold 1X Lysis buffer (1X RIPA, supplemented with 1X protease inhibitor, 1X phosphatase inhibitor, 50 mM N-Ethylmalamide) was then added to the sample, and cells were left on ice for 20 min with vortexing every 5 min for 10 s