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Lactobacillus and Bifidobacterium residing in the gastrointestinal tract possess the capacity to enzymatically convert nitrate and nitrites into nitric oxide (NO), thereby endowing the gut epithelia with a substantial reservoir of NO (Das and Ganesh, 2023)

Western blot analysis The levels of cellular proteins were quantified using Western blot analysis by utilizing primary antibodies: anti--catenin (1:1000), anti-pGSK3 (Ser9, 1:1000), anti- tGSK3 (1:1000), anti-cyclin D1 (1:1000), anti-p-Akt (1:1000), anti-tAkt (1:1000) (all from Cell Signaling Technology, Danvers, MA, USA), or anti-GAPDH (1:5000

Available at: 34 InoueKGuoRLeeMLEbrahimiRNeverovaNVCurrierJWet al

Is cadmium chloride-induced inter-Sertoli tight junction permeability barrier disruption a suitable in vitro model to study the events of junction disassembly during spermatogenesis in the rat testis