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Clean the injection site with an alcohol swab and allow to dry completely Pinch a fold of skin and subcutaneous tissue between thumb and forefinger Insert a 29 or 30 gauge insulin needle at a 45 degree angle into the pinched tissue Inject the solution slowly and steadily Withdraw the needle and apply gentle pressure if needed Rotate injection sites daily to prevent localized irritation Site Rotation Alternating between medial and lateral sides of the Achilles area prevents accumulation of scar tissue from repeated injections at the same spot

It is attributed with effects on blood vessel growth, increasing collagen or elastin synthesis

PMID: 24672108

Safety and Regulation: Since it is not approved by major regulatory bodies like the FDA for any medical use, its presence in blood when administered as a supplement or drug raises questions about safety, efficacy, and legal implications