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Animals and tissues In total, 40 male SpragueDawley rats (6 weeks old) were randomly allocated into five groups (n = 8 per group): control group, corn oil (MedChemExpress, China) injection [subcutaneously (s.c.)] + dimethyl sulfoxide (DMSO) (MedChemExpress, China) [intraperitoneally (i.p.)] + DMSO [intragastrically (i.g.)]