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This TCA cycle suppression results in a large decrease in ATP, which adversely affects all ATP-dependent proteins, including those involved in nutrient transport, mobilization, and uptake, leading to mouse death

The peak fractions were collected and incubated with ligands (either AdoCbl, MeCbl or BV) for at least 2 h at 4 C under dark conditions

It has been clearly demonstrated that altered circulating thyroid hormone is associated with various mental signs and symptoms, such as emotional disturbances (impulsiveness, irritability), cognitive deficits (impairments in memory, concentration, attention, planning, and productivity), and affective symptoms (anxiety, depression, mania), which increase the incidence of neuropsychiatric disorder like depression (111)