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Pathak, and D

The insertion technique should be executed with gentleness to avoid harming the abdominal organs

Following transfection, the cells were lysed with reporter lysis buffer (Promega), and luciferase activity was measured with the Dual-Luciferase Reporter Assay System (Promega, E1910) according to the manufacturer's protocol and quantified using a GloMax 20/20 Luminometer (Promega, USA)