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Breastfeeding and Neonatal Sleep: The Circadian Rhythms of the Breast Milk Microbiome and Metabolome and Their Impact on Global Infant Health

Science 275 : 9094 Saito M, Nishitoh H, Fujii M, Takeda K, Tobiume K, Sawada Y, Kawabata M, Miyazono K and Ichijyo H (1998) Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) 1

Tissue ROS staining To measure the ROS level of cardiac tissue, heart was embedded with optimal cutting temperature compound and placed in the refrigerator at 80C, the frozen heart was cut into 6 m slices and washed them with cold PBS