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The Phase 2 trial used subcutaneous injection into the abdomen, thigh, or upper arm on a rotating basis to minimize local reactions

DZN's inhibition of acetylcholinesterase (AChE) increases sympathetic nervous system activity and indirectly causes hepatic stress, and induction of oxidative stress (OS) leads to the generation of free radicals such as reactive oxygen species (ROS) contributes to damage cellular lipids, proteins, and DNA, leading to lipid peroxidation, nitric oxide reaction, and mitochondrial damage, which in turn affects the membrane permeability and allows the enzymes to leak into circulation

The correlation coefficients of the standard curves were above 0.99 for each primer set and 384 plate