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Similar experiments in diabetic animals indicate that grape seed extract limits the ocular blood vessel damage seen in diabetic retinopathy (degradation of the retina), which shares some pathological characteristics with AMD (Li 2008)

For example, in DR, TXNIP inhibition paradoxically suppresses autophagy (133), highlighting the tissue-specific and disease context-dependent nature of the autophagic regulation of TXNIP

Proteins were transferred to PVDF membranes and blocked with 5% non-fat milk at room temperature for 1 h

Real-time quantitative reverse transcription PCR The skin tissues from the back of mice were removed and stored at 80C