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Wu Q, Ishikawa T, Sirianni R, et al

Other contributors to this work include Premranjan Kumar, Chun Liu, James W

Cell death was shown by the change in cell viability, which was calculated as: (viable cell)% = (optical density (OD) of untreated sample/OD of treated sample) 100

the reduced dye then undergoes non-enzymic oxidation in air, so the addition of a relatively small amount of methylene blue will effectively deplete NADPH, and would be expected to stimulate any pathway that reduces NADP+ to NADPH