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Group-III: HFD and nicotine (1.5 mg/kg, b.wt, I.P) (Sinha-Hikim AP, et al ., 2017)+Vitamin-U (100 mg/kg, b.wt) (Sokmen BB, et al ., 2012)

The eluted samples were dried using a speed vacuum and re-suspended in 100 L of 200 mM HEPES buffer (pH 8.5)

Ferroptosis is a specific type of cell death triggered by the presence of ROS that rely on iron [46], it is a distinct form of controlled cell death that lacks the involvement of caspases, which are crucial in inducing apoptosis by cleaving specific intracellular substrates [48, 49]