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Immunofluorescence staining and analysis For visualization of RNH1(D210N)-GFP foci and H2AX foci, cells grown on autoclaved coverslips were washed with ice-cold PBS and pre-extracted for 5 min with CSK [25 mM HEPESNaOH (pH 7.7), 50 mM NaCl, 1 mM EDTA, 3 mM MgCl 2 , 0.3 M sucrose, 0.5% (v/v) Triton X-100] buffer on ice