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Finally, the samples were brought to dryness using an evaporation system (TurboVap LV, Biotage, Uppsala, Sweden) and resuspended in 100 L methanol-water (50/50, v/v) for UPLC-MS/MS automated injections

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The transdermal composition of claim 2 which sustains constant plasma levels of GHK-Cu or copper tripeptide in an animal for at least 24 hrs after application