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reported a decreased incidence of G6PD-d in patients presenting to the hospital for coronary artery disease, though the sample size of G6PD-d patients was again limited and the case control design has more limitations when compared to retrospective cohort studies 17

Assessment of antioxidant enzyme activities and oxidative stress markers To assess antioxidant enzyme activities and oxidative stress markers, frozen-thawed spermatozoa of each group (repeated in triplicated) were collected and centrifuged at 800g for 20 min to separate spermatozoa from seminal plasma

Then sample copy number was computed with standard curve and normalized with GAPDH