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All rats were habituated to the testing apparatus (dark-wall chamber with floor space floor 40 29 cm 2 ) for 1 week prior to the surgery

doi: 10.1016/s0034-5687(99)00016-x

Rats fed with B12 deficient diet triggered NASH through activation of pro-inflammatory pathways with an imbalanced release of IL- 1, IL- 10, and MCP1 [19]

Impact of metabolism on the stability of Tregs in the TME Owing to the unique metabolic characteristics of tumor cells, changes in nutrient availability, metabolic byproducts, and oxygen within the TME can influence the presence and function of various immune cells, including TI-Tregs