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The hormetic effect of micronutrients in general has been abundantly demonstrated, where both deficiency and excess can cause detrimental effects and physiological (relatively lower) doses are beneficial, but higher doses may do damage.26 A similar principle can be applied to antioxidants, including bioactive compounds or phytonutrients.27 Multiple antioxidant micronutrients, including vitamin C, have been shown to take on prooxidative qualities in higher dosages.28 These riskier higher dosages are unlikely to be from antioxidants sourced from whole foods in a balanced diet with intake at physiological levels and more commonly appear with supplementation

Primer sequences used were as follows: GAPDH (F: CCTCGTCCCGTAGACAAAATG, R: TGAGGTCAATGAAGGGGTCGT);ANP (F: CCGATAGATCTGCCCTCTTGAA, R: GCTGTTGCAGCCTAGTCCACT);BNP (F: GGAGGCGAGACAAGGGAGAA, R: CCAGCGGTGACAGATAAAGGAA);-MHC (F: ATGAGGAGTAGCTCTTGTGCTACC, R: CCACCTAAAGGGCTGTTGC);All primers were synthesized by RiboBio (Guangzhou, China)

Targeting DLL3: innovative strategies for tumor treatment