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For intracellular cytokine staining, the murine cells were stimulated for 5 h at 37 C with PMA (500 ng/mL) plus ionomycin (500 ng/mL), in the presence of brefeldin A and monensin

When your body metabolises them, one of the byproducts is sulphite

For multi-arm or longitudinal studies, researchers can correlate batch-specific variation with endpoint variation, something that is awkward to reconcile when reconstituting from unlabelled lyophilised vials across multiple sessions, where each reconstitution introduces fresh variables