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Briefly, 96-well ELISA microplates were coated with HGFR/c-MET Fc chimera (50 ng/well), blocked with 1% BSA, 5% sucrose, and 0.05% sodium azide in PBS, and then incubated for 2 h at 37C with HGF samples

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Its important to note that vitamin C in food is easily destroyed by heat, but since many good sources of the nutrient are fruits and vegetables, simply eating some of those foods raw is an easy way to reach the recommended intake