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The cryoprotective efficacy of L-proline has been validated across species: in sperm cryopreservation, supplementation with 2 mM L-proline improved post-thaw motility, plasma/acrosomal membrane integrity, and antioxidant capacity and reduced Caspase-3/-9 levels in goat sperm while increasing proline dehydrogenase 1 (PRODH) protein expression (Zhang et al., 2022)

Fish like salmon and trout also offer high levels

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20,189 Moreover, certain nanomaterials such as CAT-ecSNA-Cu and NP@ESCu induce cuproptosis in tumor cells and increase PD-L1 expression, though creating favorable conditions for combined anti-tumor therapy with immunotherapy