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Enhances absorption and ensures the formulation spreads smoothly across the skin

Plant Physiol 190:11171133 Paweczyk J, Brzostek A, Minias A, Pociski P, Rumijowska-Galewicz A, Strapagiel D, Zakrzewska-Czerwiska J, Dziadek J (2021) Cholesterol-dependent transcriptome remodeling reveals new insight into the contribution of cholesterol to Mycobacterium tuberculosis pathogenesis

Bumetanide treatment consisted of two 0.5 mg tablets per day for 3 months, given at 8:00 am and 4:00 pm

Protein preparation and Western blot analysis Cultured cells or flashfrozen tissues were homogenized in icecold radioimmunoprecipitation assay (RIPA) buffer (50 mM TrisHCl pH 7.6, 150 mM NaCl, 0.5% sodium deoxycholate, 1% NP40, 0.1% SDS, 2 mM EDTA) containing COMPLETE protease inhibitor cocktail (Roche) and phosphatase inhibitors (Santa Cruz Biotechnology)