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For example, it has been shown that exceeding the upper limit of selenium intake (400 g/d) can lead to selenotoxicity, but no significant symptoms of toxicity were observed when selenium was ingested in the form of SeNPs (1,600 g/d), and some selenotoxicity symptoms did not occur until even an intake of 3,200 g/d (255)

The Ni-NTA flow-through was concentrated and applied to a Superdex 200 column equilibrated with 25 mM Tris 7.5, 60 mM NaCl and 5 mM DTT

[DOI] [PMC free article] [PubMed] [Google Scholar] [84].Ni HM, Boggess N, McGill MR, Lebofsky M, Borude P, Apte U, Jaeschke H, Ding WX

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