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Recently, in locus coeruleus neurons and using chemicals activators (phorbol-12,13-dibutyrate and phorbol-12-myristate-13-acetate) or a muscarinic agonist known to activate PKC, acute or sustained desensitization of MOR induced either by morphine or [Met 5 ]-enkephalin were demonstrated to differentially required PKC activity but such effects were not inhibited by the potent PKC inhibitor staurosporine (Arttamangkul et al., 5 ]-enkephalin, the molecular mechanisms involved in MOR desensitization change during brain development