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Wet weight (mg) of liver per ml of total homogenate for each group of rats was calculated and using this value and concentration of GSH in liver cytosol fraction for each group, the GSH content of liver (moles g -1 wet weight) was determined as presented in Table 1

High pH fractionation- TMT labeled, combined, and dried samples were resuspended in 300 L of Buffer A (5% v/v MeCN, 95% v/v H 2 O, 0.1% v/v formic acid) and fractionated by centrifugation using a peptide desalting column (Pierce, #89852)

Moreover, such studies will further promote the research and development of drugs related to glutamine metabolism inhibition and thus provide more treatment options and hope for cancer patients

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