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Under homeostatic conditions, Kelch-like ECH-associated protein 1 (Keap1) binds to Nrf2 to sequester Nrf2 in the cytoplasm, promoting its degradation via the proteasome (Itoh et al., 1999)

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Cryptococcus Cryptococcosis is a life-threatening invasive fungal infection primarily caused by Cryptococcus neoformans and Cryptococcus gattii , accounting for approximately 152,000 new cases and 110,000 deaths annually, predominantly among immunocompromised individuals such as HIV/AIDS patients and organ transplant recipients (Chayakulkeeree and Perfect, 2006

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Luciferase reporter assay A luciferase reporter assay was assessed as previously described with modifications [17]

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