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The fact that CysC is sulfenylated in all OAS together with the observed persulfidation of three OAS isoforms may suggest a two-step mechanism in which sulfenylation precedes persulfidation, i.e., when sulfenic acids react with H 2 S

Subsequently, samples were washed 3 times with PBS, followed by incubation with goat anti-rabbit IgG (H + L) Alexa Fluor 647 secondary antibody (ab190589, 1/100, Abcam) or goat anti-rabbit IgG (H + L) Alexa Fluor 488 secondary antibody (ab281847, 1/100, Abcam) for 3 hours, and then washed 3 times with PBS again

D., Fisher, P