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For steady-state levels of pERK, the top ranked positive regulators were abundance of ERK, MEK, and the phosphorylation of MEK by PDK1, whereas the most important negative regulators were ERK dephosphorylation, MEK dephosphorylation, and Ras deactivation (Fig

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OGA-2KA) in this loop region in an attempt to decouple this stalk-pHAT interaction does not alter hOGA activity, indicating that this interaction is not critical for regulating the activity of hOGA or substrate selection (Supplementary Fig