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223 HIF-1 plays a pivotal role in the metabolic shift to aerobic glycolysis, known as the Warburg effect, facilitating glucose conversion to lactate despite sufficient oxygen availability

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Following incubation, the beads were washed with lysis buffer, and the proteins were eluted with GST elution buffer (75 mmol/L Tris-HCl pH 8.0, 20 mmol/L-Glutathione) and His elution buffer (50 mmol/L NaH 2 PO 4 , 300 mmol/L NaCl, 250 mmol/L imidazole, adjust pH to 8.0), respectively

Ligand binding triggers receptor autophosphorylation and recruitment of insulin receptor substrate (IRS-1, IRS-2) and Shc adapter proteins, activating the PI3KAktmTOR and RasRafMEKERK signaling cascades