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Treatment of AMs with synthetic miR-21-5p mimics or miR-NC revealed that miR-21-5p significantly reduced the intracellular bacterial counts of Actinobacillus pleuropneumoniae (APP) when compared to miR-NC treatment( Figure 4D ) through a novel mechanism: the PIK3CD gene in AMs is targeted to activate the PI3K/Akt pathway, which in turn induces autophagy, this inhibits the overproduction of ROS and activates the NLRP3 inflammasome, preventing focal death and improving bacterial clearance

However, because b-FGF can suppress tendon cell differentiation and collagen synthesis, Qiu et al (145) recommended discontinuing b-FGF in the second stage of culture to enhance further positive effects

6, 2021, article 1915

The inert matrix is typically composed of cross-linked agarose or polyacrylamide