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While ROS were also increased by day 2 post-stimulation, restoration of PPP activity and redox homeostasis using galactose by 48 h did not restore antibody production so long as glycolytic flux was suboptimal

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More molecular mechanisms of lipid metabolism in liver cancer need to be explored

Following high speed centrifugation (30,000g) to remove insoluble particles, the GuHCl solubilized protein is refolded by quickly mixing the GuHCl extract with 20 volumes of buffer containing 50 mM sodium, pH 4.5, 150 mM NaCl, 2 mM EDTA by vigorous stirring