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For the cell migration assay, 1 10 5 cells were seeded into the upper chamber of the Transwell apparatus (Costar, MA) with 100 l DMEM medium without FBS, and 600 l DMEM with 10% FBS was added to the lower chamber as a chemoattractant

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The main drawbacks of the injections are that they are not always effective, and when effective, the pain relief tends to be temporary, ranging from one week to one year

[DOI] [PMC free article] [PubMed] [Google Scholar] 88.Buzzetti E., Kalafateli M., Thorburn D., Davidson B.R., Tsochatzis E., Gurusamy K.S