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PARP inhibitors impair DNA repair via PARP enzyme blockade, inducing lethal DNA damage in homologous recombination repair (HRR)-deficient tumors (e.g., BRCA1/2-mutated PCa) (Wu et al., 2021)

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Expression and purification of recombinant proteins UBQLN1, UBQLN2, UBQLN4, HA-UBQLN2, and -syn were amplified, subcloned, and inserted into the pGEX-6p-1 vector (Cytiva)