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This difference directly impacts how both proteins are purified from cell extracts, while Ts26GST is purified chromatographically with a glutathione Sepharose-4B affinity column, Ts24GST does not bind to this resin and must be purified by ion and filtration chromatographies

From the genetics angle, there are some pediatric, congenital causes of 5-oxoprolinuria which not surprisingly involved defects in enzymes of the gamma-glutamyl cycle (in particular, mutations in glutathione synthetase and 5-oxoprolinase have been identified in these patients).