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For both techniques, the supernatant of Vero E6 cells was removed and the cells were inoculated with 50 l/well of sera-virus mixture and incubated for 1 h at room temperature under gentle shaking, allowing the infection of cells by non-neutralized viral particles

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When absorption is impaired due to a diagnosed condition such as pernicious anaemia, injections are generally the preferred and more effective option

iron is scarce, so the design is biased to maintain it