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Expression was induced with 1 mM isopropyl--D-1-thiogalactopyranoside (IPTG) at 20 C for 14 h

The experiments were assessed by observers blinded as to the treatment

After MAPK activation, the phosphorylation of MLCK is increased by a significant number of inflammatory factors (such as TNF-), subsequently leading to MLC phosphorylation and ultimately resulting in the disruption of intestinal tight junctions and an increase in intestinal permeability

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