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Conversely, murine oocyte co-culture experiments using EVs derived from follicular fluid of older women demonstrated that short-term exposure (14 h) to age-associated EV populations can exacerbate oxidative stress, mitochondrial dysfunction, and spindle abnormalities, ultimately impairing oocyte maturation (Liu et al., 2023)

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After 8 min, the drug is diluted to 0.1%, and after 15 to 25 min, almost all the active ingredients are removed from the corneal surface [38]